Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [mFluor Violet 450 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34608MFV450
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
mFluor Violet 450 SE (Excitation = 406 nm, Emission = 445 nm)
Antibody Source
Monoclonal Mouse IgG2b Kappa/IgG1 Kappa Clone # DT101+ BC199 + HMB45
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary
Immunogen
Recombinant Melanoma Marker (MART-1 + gp100) protein (DT101 & BC199); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Localization
Cytoplasmic
Specificity
This antibody cocktail recognizes two melanoma-specific proteins, which include MART-1 and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. A cocktail of these two markers is sensitive for labeling formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa/IgG1 Kappa
Scientific Data Images
Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [mFluor Violet 450 SE] [NBP2-34608MFV450] -
Melanoma Marker (MART-1 + gp100) Antibody (DT101+ BC199 + HMB45) [mFluor Violet 450 SE] [NBP2-34608MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.Applications
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Melanoma Marker (MART-1 + gp100)
Alternate Names
MART1, MART-1
Gene Symbol
MLANA
Additional Melanoma Marker (MART-1 + gp100) Products
Product Specific Notices
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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