Melanophilin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-943
Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ARHIFAKPVMAQQP corresponding to C-Terminus according to Uniprot Mouse NP_443748.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Melanophilin Antibody
![Western Blot: Melanophilin Antibody [NB300-943]](https://resources.bio-techne.com/images/products/Melanophilin-Antibody-Western-Blot-NB300-943-img0003.jpg "Western Blot: Melanophilin Antibody [NB300-943]")
Western Blot: Melanophilin Antibody [NB300-943]
Western Blot: Melanophilin Antibody [NB300-943] - Staining (0.5 ug/mL) of total melanocyte PNS (200 ug per lane) of wild-type and leaden (Mlph null) mice. The upper band may be the stacking-separating gel interface. Data kindly provided by A N Hume, Imperial College![Immunocytochemistry/ Immunofluorescence: Melanophilin Antibody [NB300-943]](https://resources.bio-techne.com/images/products/Melanophilin-Antibody-Immunohistochemistry-Paraffin-NB300-943-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Melanophilin Antibody [NB300-943]")
Immunocytochemistry/ Immunofluorescence: Melanophilin Antibody [NB300-943]
Immunocytochemistry/Immunofluorescence: Melanophilin Antibody [NB300-943] - Wild-type C57bl6 derived melanocytes were stained with (0.5 ug/mL) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.Applications for Melanophilin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.5 ug/mL
Peptide ELISA
Detection limit 1:32000
Western Blot
0.5 - 2 ug/mL
Application Notes
IF successful usage for stained mouse melanocytes is reported in scientific literature (PMID: 18559336). GST-pull down assay usage is reported in scientific literature(PMID: 18559336). WB: Approx. 80 kDa band observed in mouse Melanocyte extracts. Please note that this product may require overnight incubation of primary and high protein loads.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Melanophilin
Alternate Names
exophilin-3, l(1)-3Rk, l1Rk3, ln, melanophilin, MGC2771, MGC59733, SLAC2A, Slac-2a, SLAC2-A, Slp homolog lacking C2 domains a, Synaptotagmin-like protein 2a
Gene Symbol
MLPH
UniProt
Additional Melanophilin Products
Product Documents for Melanophilin Antibody
Product Specific Notices for Melanophilin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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