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Mena Antibody (3E6)

Novus Biologicals, part of Bio-Techne | Catalog # H00055740-M01

Novus Biologicals, part of Bio-Techne
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H00055740-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3E6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

ENAH (NP_001008493.1, 291 a.a. ~ 390 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LQAASQPAETPSQQGIVLGPLAPPPPPPLPPGPAQASVALPPPPGPPPPPPLPSTGPPPPPPPPPLPNQVPPPPPPPPAPPLPASGFFLASMSEDNRPLT

Specificity

Reacts with enabled homolog (Drosophila).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Mena Antibody (3E6)

Western Blot: Mena Antibody (3E6) [H00055740-M01]

Western Blot: Mena Antibody (3E6) [H00055740-M01]

Western Blot: Mena Antibody (3E6) [H00055740-M01] - Analysis of ENAH expression in transfected 293T cell line by ENAH monoclonal antibody (M01), clone 3E6.Lane 1: ENAH transfected lysate (Predicted MW: 66.5 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Mena Antibody (3E6) [H00055740-M01] - Detection limit for recombinant GST tagged ENAH is 3 ng/ml as a capture antibody.

Applications for Mena Antibody (3E6)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Mena

ENAH/MENA belongs to the Ena/vasodilator-stimulated phosphoprotein (VASP) family of proteins which play a role in regulating the actin cytoskeleton for the control of cell shape and movement. The Ena/VASP family of actin binding proteins includes ENAH/MENA which is the human homolog of drosophila Ena (enabled), VASP, and EVL (Ena/vasodilator-stimulated phosphoprotein-like protein). Ena/VASP proteins contain an EVH1 domain, a central proline-rich domain, and a terminal EVH2 domain. ENAH/MENA is not expressed in normal breast but has been detected and associated with breast lesions with a high risk of transformation and primary and metastatic breast cancer.

Alternate Names

ENAH, NDPP1

Entrez Gene IDs

55740 (Human)

Gene Symbol

ENAH

Additional Mena Products

Product Documents for Mena Antibody (3E6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mena Antibody (3E6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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