MERS-CoV Nucleocapsid Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10729
Key Product Details
Species Reactivity
MERS-CoV
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1038729
Product Specifications
Immunogen
Spodoptera frugiperda insect ovarian cell line Sf21-derived mouse MERS-CoV Nucleocapsid
Met1-Thr411
Accession # YP_007188586.1
Met1-Thr411
Accession # YP_007188586.1
Specificity
Detects Mouse MERS-CoV Nucleocapsid in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for MERS-CoV Nucleocapsid Antibody
Detection of MERS-CoV MERS-CoV Nucleocapsid by Western Blot.
Western blot shows recombinant MERS-CoV Nucleocapsid protein. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Mouse MERS-CoV Nucleocapsid Monoclonal Antibody (Catalog # MAB10729) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for MERS-CoV Nucleocapsid at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Detection of Nucleocapsid by Simple WesternTM.
Simple Western lane view shows recombinant MERS-CoV nucleocapsid, loaded at 0.2 mg/mL. A specific band was detected for Nucleocapsid at approximately 57 kDa (as indicated) using 25 µg/mL of Mouse Anti-MERS-CoV Nucleocapsid Monoclonal Antibody (Catalog # MAB10729) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for MERS-CoV Nucleocapsid Antibody
Application
Recommended Usage
Simple Western
25 µg/mL
Sample: Recombinant MERS-CoV nucleocapsid
Sample: Recombinant MERS-CoV nucleocapsid
Western Blot
1 µg/mL
Sample: Recombinant MERS-CoV Nucleocapsid protein
Sample: Recombinant MERS-CoV Nucleocapsid protein
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nucleocapsid
References
- Li, Y. et al. (2019) Engineering. 5:940.
- Hurst, K. R. et al. (2009) J. Virol. 83:7221.
- Wang, Y. et al. (2015) Acta. Crystallogr. F. Struct. Biol. Commun. 71:977.
- Papageorgiou, N. et al. (2016) Acta. Crystallogr. D. Struct. Biol. 72:192.
- Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
- Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
- Chang, C-K. et al.(2016) Drug Discov. Today. 21:562.
Long Name
Nucleocapsid Protein
Alternate Names
N Protein
Entrez Gene IDs
Gene Symbol
N
UniProt
Additional Nucleocapsid Products
Product Documents for MERS-CoV Nucleocapsid Antibody
Product Specific Notices for MERS-CoV Nucleocapsid Antibody
For research use only
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