Mesothelin Antibody (CL13957)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24590
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL13957
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for Mesothelin Antibody (CL13957)
Immunogen
This antibody was designed to target the recombinant protein Mesothelin, with the precise immunogen sequence being withheld for proprietary reasons.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Mesothelin Antibody (CL13957)
Immunohistochemistry-Paraffin: Mesothelin Antibody (CL13957) [NBP3-24590]
Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Mesothelin Antibody (CL13957) [NBP3-24590]
Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Mesothelin Antibody (CL13957) [NBP3-24590]
Staining of human ovarian cancer (papillary carcinoma) shows strong membranous positivity in tumor cells.
Applications for Mesothelin Antibody (CL13957)
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Mesothelin
Alternate Names
CAK1, MPF, MSLN, SMR
Gene Symbol
MSLN
Additional Mesothelin Products
Product Documents for Mesothelin Antibody (CL13957)
Product Specific Notices for Mesothelin Antibody (CL13957)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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