Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # MSLN/2131

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Mesothelin Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary) (Uniprot: Q13421)

Localization

Cell Surface (Secreted)

Marker

Mesothelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-79724).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Mesothelin Antibody (MSLN/2131) - Azide and BSA Free

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858]

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858]

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Mesothelin Antibody (MSLN/2131).
Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858]

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858]

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858] - Formalin-fixed, paraffin-embedded Mouse Lung stained with Mesothelin Antibody (MSLN/2131).
Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858]

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858]

Immunohistochemistry-Paraffin: Mesothelin Antibody (MSLN/2131) - Azide and BSA Free [NBP2-79858] - Formalin-fixed, paraffin-embedded human Lung Mesothelioma stained with Mesothelin Antibody (MSLN/2131).
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Applications for Mesothelin Antibody (MSLN/2131) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Mesothelin

MSLN, also known as Mesothelin, has 4 isoforms, a 630 amino acid isoform that is 69 kDa, a 622 amino acid isoform that is 68 kDa, a 656 amino acid isoform that is 71 kDa, and a 621 amino acid isoform that is 68 kDa, expressed in lung, heart, placenta kidney, and overexpressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas. Membrane-anchored forms may play a role in cellular adhesion and megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. Current research is being performed on several diseases and disorders including squamous cell carcinoma, ovarian cancer, carcinoma, malignant biphasic mesothelioma, bile duct adenoma, malignant mesothelioma, malignant pleural mesothelioma, gastrointestinal stromal tumor, renal clear cell carcinoma, epithelioid sarcoma, benign mesothelioma, pancreatic ductal adenocarcinoma, asbestosis, pancreatic ductal carcinoma, thymic carcinoma, acute myeloid leukemia, epithelial ovarian cancer, myeloid leukemia, thoracic cancer, and pancreatitis. This protein has also shown an interaction with MUC16 and RSG1 in cell adhesion and pancreas development processes and pathways.

Alternate Names

CAK1, MPF, MSLN, SMR

Gene Symbol

MSLN

Additional Mesothelin Products

Product Documents for Mesothelin Antibody (MSLN/2131) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Mesothelin Antibody (MSLN/2131) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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