mGluR1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-123

Novus Biologicals, part of Bio-Techne
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NB300-123
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all mGluR1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Accession # P23385

Specificity

Specific for endogenous levels of the ~125 kDa monomer and the ~250 kDa mGluR1a dimer. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mGluR1 Antibody

Western Blot: mGluR1 Antibody [NB300-123]

Western Blot: mGluR1 Antibody [NB300-123]

Western Blot: mGluR1 Antibody [NB300-123] - 10 ug of HEK 293 cells expression. As shown in the autoradiograph, the mGluR1a antibody is specific for the ~125k monomer and the ~250k dimer of mGluR1a (Lane A). The mGluR1a antibody shows no reactivity towards mGluR5 (Lane B).
Western Blot: mGluR1 Antibody [NB300-123]

Western Blot: mGluR1 Antibody [NB300-123]

Western Blot: mGluR1 Antibody [NB300-123] - 10 ug of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125k monomer and the ~250k dimer of mGluR1a. The mGluR1a antibody shows no reactivity toward mGluR5.

Applications for mGluR1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500

Western Blot

1:1000
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 26924225).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: mGluR1

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GLUR1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

24414 (Rat)

Gene Symbol

GRM1

UniProt

P23385 (Rat)

Additional mGluR1 Products

Product Documents for mGluR1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for mGluR1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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