Methionyl tRNA synthetase Antibody (5G5)
Novus Biologicals, part of Bio-Techne | Catalog # H00004141-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 5G5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MARS (NP_004981, 801 a.a. ~ 899 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKK
Localization
Cytoplasmic
Specificity
MARS - methionine-tRNA synthetase
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Methionyl tRNA synthetase Antibody (5G5)
![Western Blot: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01]](https://resources.bio-techne.com/images/products/Methionyl-tRNA-synthetase-Antibody-5G5-Western-Blot-H00004141-M01-img0006.jpg "Western Blot: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01]")
Western Blot: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01]
Western Blot: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01] - MARS monoclonal antibody (M01), clone 5G5 Analysis of MARS expression in HepG2.![Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01]](https://resources.bio-techne.com/images/products/Methionyl-tRNA-synthetase-Antibody-5G5-Immunocytochemistry-Immunofluorescence-H00004141-M01-img0004.jpg "Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01]")
Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01]
Immunocytochemistry/Immunofluorescence: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01] - Analysis of monoclonal antibody to MARS on HepG2 cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: Methionyl tRNA synthetase Antibody (5G5) [H00004141-M01] - Detection limit for recombinant GST tagged MARS is 0.03 ng/ml as a capture antibody.
Applications for Methionyl tRNA synthetase Antibody (5G5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Methionyl tRNA synthetase
Alternate Names
cytosolic methionyl-tRNA synthetase, EC 6.1.1.10, FLJ35667, methionine tRNA ligase 1, cytoplasmic, Methionine--tRNA ligase, methionyl-tRNA synthetase, methionyl-tRNA synthetase, cytoplasmic, MetRS, MRS, MTRNS
Entrez Gene IDs
4141 (Human)
Gene Symbol
MARS1
OMIM
156560 (Human)
UniProt
Additional Methionyl tRNA synthetase Products
Product Documents for Methionyl tRNA synthetase Antibody (5G5)
Product Specific Notices for Methionyl tRNA synthetase Antibody (5G5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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