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Methionyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33016

Novus Biologicals, part of Bio-Techne
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NBP1-33016

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%), Monkey (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Methionyl tRNA synthetase. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (97%), Xenopus laevis (81%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Methionyl tRNA synthetase Antibody

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016]

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016]

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-33016]

Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-33016]

Immunocytochemistry/Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-33016] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MARS protein stained by MARS antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.
Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016]

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016]

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-33016] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MARS antibody [N3C2], Internal diluted at 1:1000.

Applications for Methionyl tRNA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Methionyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases.

Alternate Names

cytosolic methionyl-tRNA synthetase, EC 6.1.1.10, FLJ35667, methionine tRNA ligase 1, cytoplasmic, Methionine--tRNA ligase, methionyl-tRNA synthetase, methionyl-tRNA synthetase, cytoplasmic, MetRS, MRS, MTRNS

Gene Symbol

MARS1

Additional Methionyl tRNA synthetase Products

Product Documents for Methionyl tRNA synthetase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Methionyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.