Methionyl tRNA synthetase Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87730

Novus Biologicals, part of Bio-Techne
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NBP1-87730
NBP1-87730-25ul
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Methionyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methionyl tRNA synthetase Antibody

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP1-87730]

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP1-87730]

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP1-87730] - Analysis in human testis and skeletal muscle tissues using NBP1-87730 antibody. Corresponding Methionyl tRNA synthetase RNA-seq data are presented for the same tissues.
Western Blot: Methionyl tRNA synthetase Antibody [NBP1-87730]

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-87730]

Western Blot: Methionyl tRNA synthetase Antibody [NBP1-87730] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MARS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-87730]

Immunocytochemistry/ Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-87730]

Immunocytochemistry/Immunofluorescence: Methionyl tRNA synthetase Antibody [NBP1-87730] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
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Applications for Methionyl tRNA synthetase Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Methionyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases.

Alternate Names

cytosolic methionyl-tRNA synthetase, EC 6.1.1.10, FLJ35667, methionine tRNA ligase 1, cytoplasmic, Methionine--tRNA ligase, methionyl-tRNA synthetase, methionyl-tRNA synthetase, cytoplasmic, MetRS, MRS, MTRNS

Gene Symbol

MARS1

Additional Methionyl tRNA synthetase Products

Product Documents for Methionyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Methionyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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