Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Methionyl tRNA synthetase Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 175 to 225 of human Methionyl-tRNA Synthetase using the numbering given in entry NP_004981.2 (GeneID 4141).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Methionyl tRNA synthetase Antibody

Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312]

Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312]

Western Blot: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22312 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunohistochemistry-Paraffin: Methionyl tRNA synthetase Antibody [NBP2-22312] - FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS (NBP2-22312) left image and (NBP2-22313) right image, used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).
Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312]

Immunoprecipitation: Methionyl tRNA synthetase Antibody [NBP2-22312] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22312 used for IP at 6 ug/mg lysate. MARS was also immunoprecipitated by rabbit anti-MARS antibody NBP2-22313. For blotting immunoprecipitated MARS, NBP2-22312 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for Methionyl tRNA synthetase Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Methionyl tRNA synthetase

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases.

Alternate Names

cytosolic methionyl-tRNA synthetase, EC 6.1.1.10, FLJ35667, methionine tRNA ligase 1, cytoplasmic, Methionine--tRNA ligase, methionyl-tRNA synthetase, methionyl-tRNA synthetase, cytoplasmic, MetRS, MRS, MTRNS

Gene Symbol

MARS1

UniProt

NP_004981.2 (Human)

Additional Methionyl tRNA synthetase Products

Product Documents for Methionyl tRNA synthetase Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Methionyl tRNA synthetase Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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