mGluR1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-39033

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Mouse, Rat

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all mGluR1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide comprising residues 1159-1171 [SSVPSSPVSESVL] of the human mGluR1 protein.

Localization

Cell membrane

Specificity

Glutamate Receptor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

132.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for mGluR1 Antibody - BSA Free

Western Blot: mGluR1 AntibodyBSA Free [NB110-39033]

Western Blot: mGluR1 AntibodyBSA Free [NB110-39033]

Western Blot: mGluR1 Antibody [NB110-39033] - WB detection of GluR1 in 20 ug of human brain lysate (lane 2) with GluR1 polyclonal antibody at 1:1000 dilution followed by AP-conjugated secondary at 1:5000 dilution. Lane 1 represents the MW marker.
Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB110-39033]

Immunocytochemistry/ Immunofluorescence: mGluR1 Antibody - BSA Free [NB110-39033]

Immunocytochemistry/Immunofluorescence: mGluR1 Antibody [NB110-39033] - Primary Rat Cortical Neurons, image courtesy of customer.
Immunohistochemistry-Frozen: mGluR1 Antibody - BSA Free [NB110-39033]

Immunohistochemistry-Frozen: mGluR1 Antibody - BSA Free [NB110-39033]

Immunohistochemistry-Frozen: mGluR1 Antibody [NB110-39033] - Immunofluorescence labeling for GluR1 in rat brain (cingulate cortex). GluR1 detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color). Working dilution is 1:100-1:500.

Applications for mGluR1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:100-1:500

Western Blot

1:500-1:1000
Application Notes
This metabotropic Glutamate Receptor 1a antibody is useful for Western Blot, Immunohistochemistry-Frozen and Immunohistochemistry-paraffin applications. Use in Immunocytochemistry/immunofluorescence was reported in scientific literature (PMID: 19688350) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB110-39033 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: mGluR1

The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GRIA1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14810 (Mouse); 24408 (Rat)

Gene Symbol

GRM1

UniProt

Q13255

Additional mGluR1 Products

Product Documents for mGluR1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for mGluR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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