MGMT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68885

Novus Biologicals, part of Bio-Techne
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NBP2-68885
NBP2-68885-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MGMT Antibodies »

Product Summary for MGMT Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVEVPAPAAVLGGPEPLMQCTAWLNAYFHQPE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MGMT Antibody

Western Blot: MGMT Antibody [NBP2-68885]

Western Blot: MGMT Antibody [NBP2-68885]

Western Blot: MGMT Antibody [NBP2-68885] - Analysis in human cell line RT-4.
Immunohistochemistry: MGMT Antibody [NBP2-68885]

Immunohistochemistry: MGMT Antibody [NBP2-68885]

Immunohistochemistry: MGMT Antibody [NBP2-68885] - Immunohistochemical staining of human testis shows nuclear positivity of varying intensity in cells in seminiferous ducts.

Applications for MGMT Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
Recommended conditions for IHC,Retrieval method: HIER pH6
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MGMT

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Long Name

O-6 Methylguanine-DNA Methyltransferase

Alternate Names

6-O-methylguanine-DNA methyltransferase, EC 2.1.1.63, methylated-DNA--protein-cysteine methyltransferase, methylguanine-DNA methyltransferase, O-6-methylguanine-DNA methyltransferase, O6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase

Gene Symbol

MGMT

Additional MGMT Products

Product Documents for MGMT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MGMT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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