Key Product Details

Species Reactivity

Validated:

Mouse

Applications

Flow Cytometry, Immunoprecipitation

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 2G5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MHC Class I Antibodies »

Product Summary for MHC Class I Antibody (2G5) [DyLight 405]

Immunogen

Purified H-2Kb and H-2Db MHC-I molecules

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Chinese Hamster reactivity reported in scientific literature (PMID: 30441765).

Specificity

NB100-65938 recognizes a monomorphic epitope on the mouse MHC class I antigen. The epitope recognized by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognize a polymorphic epitope of canine MHC class 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for MHC Class I Antibody (2G5) [DyLight 405]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MHC Class I

The antigenic agglomerate major histocompatibility complex (MHC) class I molecules present antigenic peptides to CD8-positive T cells. It is now recognized that the products of the MHC genes are antigen-presenting molecules (APM) designed for the presentation of antigen fragments (peptides) to the T-cell receptor, thus participating in the immune response (1). Recently shown is a mechanism of antigen acquisition for cross-presentation that couples the antigen presentation system of two adjacent cells and is lost in most tumours: gap-junction-mediated intercellular peptide coupling for presentation by bystander MHC class I molecules and transfer to professional antigen presenting cells for cross-priming (2). The mammalian vomeronasal organ detects social information about gender, status, and individuality. It has been shown that small peptides that serve as ligands for major histocompatibility complex (MHC) class I molecules function also as sensory stimuli for a subset of vomeronasal sensory neurons located in the basal Gao- and V2R receptor-expressing zone of the vomeronasal epithelium (3).

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (2G5) [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MHC Class I Antibody (2G5) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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