Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # ER-HR52

Concentration

1.0 mg/ml

View all MHC Class I Antibodies »

Product Specifications

Immunogen

Adherent FI (CBAXBL) bone marrow stromal cells

Localization

Cell Membrane; Type I membrane protein.

Specificity

NB100-64952 recognizes a polymorphic determinant on the murine MHC (major histocompatibility complex) class I molecule. In the mouse, the MHC class I molecule is expressed on the cell surface of all nucleated cells but expression levels may differ according to cell type. Clone ER-MP52 specifically recognizes mouse strains with the haplotypes H-2b,w16 and H-2d,p,q. Mouse strains with the haplotypes H-2f,r,s,w17,w23,w27 show weak reactivity with this antibody.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for MHC Class I Antibody (ER-HR52)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:10-1:500
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 29195074).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MHC Class I

MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.

Alternate Names

DKFZp686P19218, EA1.2, EA2.1, HLA class I histocompatibility antigen, alpha chain E, HLA class I histocompatibility antigen, E alpha chain, HLA-6.2, HLAE, lymphocyte antigen, major histocompatibility complex, class I, E, MHC, MHC class I antigen E, MHC HLA-E alpha-1, MHC HLA-E alpha-2.1, QA1

Entrez Gene IDs

14972 (Mouse)

Gene Symbol

HLA-E

Additional MHC Class I Products

Product Documents for MHC Class I Antibody (ER-HR52)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MHC Class I Antibody (ER-HR52)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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