MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21789MFV610
![MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]](https://resources.bio-techne.com/images/products/nbp2-21789mfv610_rat-mhc-class-ii-mab-m5-114-mfluor-violet-610-se-672023994326.png "MHC Class II Antibody (M5/114) [mFluor Violet 610 SE] [NBP2-21789MFV610] -")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Applications
CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
mFluor Violet 610 SE (Excitation = 421 nm, Emission = 613 nm)
Antibody Source
Monoclonal Rat IgG2B Clone # M5/114
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]
Immunogen
Activated C57BL/6 mouse spleen cells
Reactivity Notes
Use in Rat reported in scientific literature (PMID:34166814).
Specificity
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]
MHC Class II Antibody (M5/114) [mFluor Violet 610 SE] [NBP2-21789MFV610] -
MHC Class II Antibody (M5/114) [mFluor Violet 610 SE] [NBP2-21789MFV610] - Vial of mFluor Violet 610 conjugated antibody. mFluor Violet 610 is optimally excited at 421 nm by the Violet laser (405 nm) and has an emission maximum of 613 nm.Applications for MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Functional
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Frozen
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: MHC Class II
Alternate Names
class II histocompatibility antigen, M alpha chain, D6S222E, DMA, HLA class II histocompatibility antigen, DM alpha chain, major histocompatibility complex, class II, DM alpha, MHC class II antigen DMA, Really interesting new gene 6 protein, RING6HLADM
Gene Symbol
HLA-DMA
Additional MHC Class II Products
Product Documents for MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]
Product Specific Notices for MHC Class II Antibody (M5/114) [mFluor Violet 610 SE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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