MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43312
![Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0007.jpg "Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]")
![Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0008.jpg "Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]")
![Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0009.jpg "Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Flow Cytometry, IF/IHC, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2b Kappa Clone # M5/114.15.2
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
The immunogen for this antibody was MHC Class II.
Reactivity Notes
Use in Human reported in scientific literature (PMID:29990503).
Specificity
The monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2b Kappa
Scientific Data Images for MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free
Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]
Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the PE conjugate of NBP1-43312. Analysis of mouse splenocyte suspensions using MHC Class II PE and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.![Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0001.jpg "Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]")
Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]
Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Staining of C57BL/6 splenocytes with 0.06 ug of Rat IgG2b K Isotype Control Purified (cat. NBP1-43323) (open histogram) or 0.06 ug of Anti-Mouse MHC Class II (I-A/I-E) Purified (filled histogram) followed by Anti-Rat IgG FITC (cat. NBP1-69751). Total viable cells were used for analysis.![Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]](https://resources.bio-techne.com/images/products/MHC-Class-II-Antibody-M5-114-15-2-Flow-Cytometry-NBP1-43312-img0006.jpg "Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]")
Flow Cytometry: MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free [NBP1-43312]
Flow Cytometry: MHC Class II Antibody (M5/114.15.2) [NBP1-43312] - Analysis using the Allophycocyanin conjugate of NBP1-43312. Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC and 0.015 micrograms of Rat IgG2b ? Isotype Control APC (left) or 0.015 micrograms of Anti-Mouse MHC Class II (I-A/I-E) APC (right).Applications for MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free
Application
Recommended Usage
Flow Cytometry
< 0.125 ug/10^6 cells
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 0.125 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MHC class II (I-A/I-E)
Long Name
Major Histocompatibility Complex Class II IA and IE
Alternate Names
H2-Ea, MHC class II IA/IE
Gene Symbol
H2-Ea
Additional MHC class II (I-A/I-E) Products
Product Documents for MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free
Product Specific Notices for MHC class II (I-A/I-E) Antibody (M5/114.15.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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