MICA Antibody (MICA/4442) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14208
![Protein Array: MICA Antibody (MICA/4442) - Azide and BSA Free [NBP3-14208]](https://resources.bio-techne.com/images/products/MICA-Antibody-MICA-4442-Azide-and-BSA-Free-Protein-Array-NBP3-14208-img0001.jpg "Protein Array: MICA Antibody (MICA/4442) - Azide and BSA Free [NBP3-14208]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # MICA/4442
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for MICA Antibody (MICA/4442) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa1-200) of human MICA (exact sequence is proprietary) (Uniprot: Q29983 )
Localization
Cell surface, Cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13826).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MICA Antibody (MICA/4442) - Azide and BSA Free
Protein Array: MICA Antibody (MICA/4442) - Azide and BSA Free [NBP3-14208]
Protein Array: MICA Antibody (MICA/4442) - Azide and BSA Free [NBP3-14208] - Analysis of Protein Array containing >19,000 full-length human proteins using MICA Antibody (MICA/4442).
MICA Antibody (MICA/4442)
Formalin-fixed, paraffin-embedded human stomach stained with MICA antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
MICA Antibody (MICA/4442)
Formalin-fixed, paraffin-embedded human kidney stained with MICA antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for MICA Antibody (MICA/4442) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: MICA
Long Name
MHC Class I-related Protein A
Alternate Names
FLJ60820, MGC111087, PERB11.1
Gene Symbol
MICA
Additional MICA Products
Product Documents for MICA Antibody (MICA/4442) - Azide and BSA Free
Product Specific Notices for MICA Antibody (MICA/4442) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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