Microsomal Glutathione S-transferase 1 Antibody (14H2-R)

Western Blot: Microsomal Glutathione S-transferase 1 Antibody (14H2-R) [NBP3-32594]

Western Blot: Microsomal Glutathione S-transferase 1 Antibody (14H2-R) [NBP3-32594] - Western blot analysis of Microsomal Glutathione S-transferase 1 on different lysates with Mouse anti-Microsomal Glutathione S-transferase 1 antibody (NBP3-32594) at 1/1,000 dilution.

Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: U-937 cell lysate
Lane 5: MCF7 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 18 kDa
Observed band size: 15 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32594) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Microsomal Glutathione S-transferase 1 Antibody (14H2-R) [NBP3-32594]

Immunohistochemistry: Microsomal Glutathione S-transferase 1 Antibody (14H2-R) [NBP3-32594] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Microsomal Glutathione S-transferase 1 antibody (NBP3-32594) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32594) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.