MIER2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47372

Novus Biologicals, part of Bio-Techne
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NBP2-47372
NBP2-47372-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MIER2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDELLALYGYEASDPISDRE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIER2 Antibody

Western Blot: MIER2 Antibody [NBP2-47372]

Western Blot: MIER2 Antibody [NBP2-47372]

Western Blot: MIER2 Antibody [NBP2-47372] - Analysis in control (vector only transfected HEK293T lysate) and MIER2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MIER2 Antibody [NBP2-47372]

Immunocytochemistry/ Immunofluorescence: MIER2 Antibody [NBP2-47372]

Immunocytochemistry/Immunofluorescence: MIER2 Antibody [NBP2-47372] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: MIER2 Antibody [NBP2-47372]

Immunohistochemistry-Paraffin: MIER2 Antibody [NBP2-47372]

Immunohistochemistry-Paraffin: MIER2 Antibody [NBP2-47372] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for MIER2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIER2

Transcriptional repressor

Alternate Names

KIAA1193mesoderm induction early response protein 2, mesoderm induction early response 1, family member 2, Mi-er2

Gene Symbol

MIER2

Additional MIER2 Products

Product Documents for MIER2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MIER2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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