MIG2/Kindlin-2 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36541
![Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541]](https://resources.bio-techne.com/images/products/MIG2-Kindlin-2-Antibody-Western-Blot-NBP2-36541-img0001.jpg "Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for MIG2/Kindlin-2 Antibody
Immunogen
The immunogen recognized by the antibody maps to a region between residue 175 to 225 of human Kindlin-2 using the numbering given in entry NP_006823.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MIG2/Kindlin-2 Antibody
Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541]
Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541]](https://resources.bio-techne.com/images/products/MIG2-Kindlin-2-Antibody-Immunoprecipitation-NBP2-36541-img0002.jpg "Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541]")
Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541]
Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated Kindlin-2, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for MIG2/Kindlin-2 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MIG2
Long Name
Mitogen Inducible Gene 2
Alternate Names
FERMT2, KIND2, Kindlin-2, PLEKHC1
Gene Symbol
FERMT2
UniProt
Additional MIG2 Products
Product Documents for MIG2/Kindlin-2 Antibody
Product Specific Notices for MIG2/Kindlin-2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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