Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MIG2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by the antibody maps to a region between residue 175 to 225 of human Kindlin-2 using the numbering given in entry NP_006823.1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIG2/Kindlin-2 Antibody

Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541]

Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541]

Western Blot: MIG2/Kindlin-2 Antibody [NBP2-36541] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Kindlin-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541]

Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541]

Immunoprecipitation: MIG2/Kindlin-2 Antibody [NBP2-36541] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated Kindlin-2, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for MIG2/Kindlin-2 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MIG2

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites

Long Name

Mitogen Inducible Gene 2

Alternate Names

FERMT2, KIND2, Kindlin-2, PLEKHC1

Gene Symbol

FERMT2

UniProt

NP_006823.1 (Human)

Additional MIG2 Products

Product Documents for MIG2/Kindlin-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MIG2/Kindlin-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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