MINA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89863
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: MPKKAKPTGSGKEEGPAPCKQMKLEAAGGPSALNFDSPSSLFESLISPIKTETFFKEFWEQKPLLIQRDDPALATYYGSLFKLTDLKSLCSRGMYYGRDVNVCRCVNGKKKVLNKDGKAHFLQLRKDFDQKRATIQFHQPQRFKDELW
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (81%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MINA Antibody
Western Blot: MINA Antibody [NBP1-89863]
Western Blot: MINA Antibody [NBP1-89863] - Analysis in control (vector only transfected HEK293T lysate) and MINA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunocytochemistry/ Immunofluorescence: MINA Antibody [NBP1-89863]
Immunocytochemistry/Immunofluorescence: MINA Antibody [NBP1-89863] - Staining of human cell line U-2 OS shows localization to nucleus and nucleoli.Immunohistochemistry-Paraffin: MINA Antibody [NBP1-89863]
Immunohistochemistry-Paraffin: MINA Antibody [NBP1-89863] - Staining of human thyroid gland shows nuclear positivity in glandular cells.Applications for MINA Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MINA
Long Name
Myc-induced Nuclear Antigen
Alternate Names
MDIG, Mina53, NO52
Gene Symbol
RIOX2
Additional MINA Products
Product Documents for MINA Antibody
Product Specific Notices for MINA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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