Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Mind Bomb 2/MIB2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 925 and 975 of human mindbomb homolog 2 (Skeletrophin) using the numbering given in entry NP_543151.1 (GeneID 142678).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Mind Bomb 2/MIB2 Antibody

Western Blot: Mind Bomb 2/MIB2 Antibody [NB100-68224]

Western Blot: Mind Bomb 2/MIB2 Antibody [NB100-68224]

Western Blot: Mind Bomb 2/MIB2 Antibody [NB100-68224] - Detection of Human MIB2/Skeletrophin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIB2/Skeletrophin antibody NB100-68224 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Mind Bomb 2/MIB2 Antibody

Application
Recommended Usage

Western Blot

1:1000 - 1:5000
Application Notes
Immunoprecipitation Not recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Mind Bomb 2/MIB2

MIB2/Skeletrophin is an actin-binding protein that bears two MIB/HERC2 domains, a ZZ-type zinc-finger domain, nine ankyrin repeats, and two RING-finger domains. The C-terminal RING domain has been found to have E3 ubiquitin ligase activity that mediates ubiquitination of the intracellular regions of the Notch receptor and its ligands to regulate Notch signaling. MIB2/Skeletrophin appears to be important to muscle development and may function as a suppressor of melanoma invasion.

Long Name

Mindbomb Homolog 2

Alternate Names

MIB2, Mind Bomb-2, Novelzin, SKD, Skeletrophin, ZZANK1, ZZZ5

Entrez Gene IDs

142678 (Human)

Gene Symbol

MIB2

UniProt

NP_543151.1 (Human)

Additional Mind Bomb 2/MIB2 Products

Product Documents for Mind Bomb 2/MIB2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mind Bomb 2/MIB2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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