Mitochondria Antibody (MTC02/2860R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07309
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02/2860R) [NBP3-07309]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-MTC02-2860R-Immunohistochemistry-Paraffin-NBP3-07309-img0001.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02/2860R) [NBP3-07309]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse (Negative), Rat (Negative)
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # MTC02/2860R
Concentration
0.2 mg/ml
Product Summary for Mitochondria Antibody (MTC02/2860R)
Immunogen
Semi-Purified mitochondrial preparation
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Mitochondria in cytoplasm
Specificity
This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial monoclonal antibody has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this monoclonal antibody facilitates the classification of salivary tumors.
Marker
Marker for Human Cells, Granular RCC's & Salivary Tumors
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Mitochondria Antibody (MTC02/2860R)
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02/2860R) [NBP3-07309]
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02/2860R) [NBP3-07309] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Rabbit Recombinant Monoclonal Antibody (MTC02/2860R).Applications for Mitochondria Antibody (MTC02/2860R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Mitochondria
Additional Mitochondria Products
Product Documents for Mitochondria Antibody (MTC02/2860R)
Product Specific Notices for Mitochondria Antibody (MTC02/2860R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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