Mitochondria Antibody (MTC02)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32982
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat (Negative)
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MTC02
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Semi-Purified mitochondrial preparation
Reactivity Notes
Does not react with Rat.
Localization
Mitochondria in cytoplasm
Specificity
This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This monoclonal antibody recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
Marker
Marker for Human Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34686)
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for Mitochondria Antibody (MTC02)
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02) [NBP2-32982]
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02) [NBP2-32982] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Antibody (MTC02).Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02) [NBP2-32982]
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC02) [NBP2-32982] - Formalin-fixed, paraffin-embedded human Tonsil stained with Mitochondria Monoclonal Antibody (MTC02).Applications for Mitochondria Antibody (MTC02)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Frozen
1:200-1:400
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by heating tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Mitochondria
Additional Mitochondria Products
Product Documents for Mitochondria Antibody (MTC02)
Product Specific Notices for Mitochondria Antibody (MTC02)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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