Mitochondria Antibody (MTC719) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34687
![Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-MTC719-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34687-img0002.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MTC719
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Mitochondria Antibody (MTC719) - Azide and BSA Free
Immunogen
Mitochondrial fraction of HeLa cells
Reactivity Notes
Does not react with Insects or Bacteria.
Localization
Mitochondria in cytoplasm
Specificity
This monoclonal antibody recognizes a 60kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-32873).
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for Mitochondria Antibody (MTC719) - Azide and BSA Free
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687] - Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Monoclonal Antibody (MTC719).![Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-MTC719-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34687-img0001.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]")
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719).![Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]](https://resources.bio-techne.com/images/products/Mitochondria-Antibody-MTC719-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34687-img0003.jpg "Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]")
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687]
Immunohistochemistry-Paraffin: Mitochondria Antibody (MTC719) - Azide and BSA Free [NBP2-34687] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Monoclonal Antibody (MTC719).Applications for Mitochondria Antibody (MTC719) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5-1ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.25-0.5ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Mitochondria
Additional Mitochondria Products
Product Documents for Mitochondria Antibody (MTC719) - Azide and BSA Free
Product Specific Notices for Mitochondria Antibody (MTC719) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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