Mitofusin 1 Antibody (11G8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26143
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 11G8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Mitofusin 1 Antibody (11G8)
Immunogen
A synthesized peptide derived from Human Mitofusin 1 [UniProt Q8IWA4]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mitofusin 1 Antibody (11G8)
Flow Cytometry: Mitofusin 1 Antibody (11G8) [NBP3-26143] -
Flow Cytometry: Mitofusin 1 Antibody (11G8) [NBP3-26143] - Overlay Peak curve showing HepG2 cells stained with Mitofusin 1 Antibody (11G8) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for Mitofusin 1 Antibody (11G8)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: Mitofusin 1
Mitochondrial fusion occurs in many cell types and is a fundamental component of mitochondria morphology, which is balanced between fusion and fission. Mitochondrial dysfunction is a major cause of neuronal death in degenerative disorders, such as Parkinson's disease, and can be triggered by an imbalance in the dynamic equilibrium between mitochondrial fission and fusion.
Alternate Names
hfzo1, hfzo2, MFN1
Gene Symbol
MFN1
Additional Mitofusin 1 Products
Product Documents for Mitofusin 1 Antibody (11G8)
Product Specific Notices for Mitofusin 1 Antibody (11G8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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