Mitofusin 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51841

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Ovine

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Mitofusin 1 Antibodies »

Product Specifications

Immunogen

A genomic peptide made to an N-terminal region of human Mitofusin-1 (within residues 5-100). [Swiss-Prot Q8IWA4]

Localization

Mitochondrion outer membrane; Multi-pass membrane protein.

Marker

Mitochondrial Fusion Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mitofusin 1 Antibody - BSA Free

Western Blot: Mitofusin 1 AntibodyBSA Free [NBP1-51841]

Western Blot: Mitofusin 1 AntibodyBSA Free [NBP1-51841]

Western Blot: Mitofusin 1 Antibody [NBP1-51841] - Densitometric analyses of protein expression levels relative to the sedentary group with representative image of western blot are shown. n = 5-6/group. Values are expressed by mean +/- SEM expressed as fold change relative to the sedentary group. * P < 0.05, significantly different from the sedentary group. Black bar: fetal hearts from sedentary dams; gray bar: fetal hearts from exercised dams. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350185/) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunocytochemistry/ Immunofluorescence: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunocytochemistry/Immunofluorescence: Mitofusin 1 Antibody [NBP1-51841] - Mfn-1 expressed in mouse neural stem cells. Image from verified customer review.
Immunohistochemistry: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunohistochemistry: Mitofusin 1 Antibody - BSA Free [NBP1-51841]

Immunohistochemistry: Mitofusin 1 Antibody [NBP1-51841] - IHC staining of Mfn1 in mouse intestine.
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Applications for Mitofusin 1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

reported in scientific literature
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reviewed Applications

Read 1 review rated 4 using NBP1-51841 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mitofusin 1

Mitochondria are dynamic organelles that continuously divide and fuse, and their dynamics/distribution is critical for their role in bioenergetics and cell survival. Mitochondrial fusion process is mediated by mitofusin 1 (MFN1) and mitofusin 2 (MFN2) proteins in the outer membrane as well as OPA1 in the inner mitochondrial membrane. MFN1 is an essential transmembrane GTPase critical to mitochondrial fusion; acts independently of the cytoskeleton, and its overexpression induces formation of mitochondrial networks. MFN1 forms homo-/-heteromultimers (with MFN2), and the multimerization has been proposed to be essential for mitochondrion fusion. MFN1 interacts with VAT1 and is ubiquitinated by MARCH5. A mutation in the GTPase domain disrupts the mitochondrial fusion activity of MFN1/MFN2 and loss of MFN1/2 leads to mitochondrial dysfunction mediated embryonic lethality in mice. MFN proteins have been reported to regulate mitochondrial membrane potential, while silencing of MFN1/2 resulte in mitochondrial fragmentation and increased apoptotic sensitivity.

Alternate Names

hfzo1, hfzo2, MFN1

Entrez Gene IDs

55669 (Human)

Gene Symbol

MFN1

UniProt

Q8IWA4

Additional Mitofusin 1 Products

Product Documents for Mitofusin 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Mitofusin 1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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