Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S153-5

Concentration

1 mg/ml

View all Mitofusin 2 Antibodies »

Product Summary for Mitofusin 2 Antibody (S153-5)

Immunogen

Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Mouse and Rat.

Localization

Mitochondrion , Mitochondrion Outer Membrane

Specificity

Approx 55% identity with Mitofusin-1.Detects approx 90kDa. No cross-reactivity against Mitofusin-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Mitofusin 2 Antibody (S153-5)

Western Blot: Mitofusin 2 Antibody (S153-5) [NBP2-42188]

Western Blot: Mitofusin 2 Antibody (S153-5) [NBP2-42188]

Western Blot: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa Mitofusin 2 protein using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5 (NBP2-42188). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody (NBP2-42188) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.
Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188]

Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188]

Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone S153-5 (NBP2-42188). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody (NBP2-42188) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Mitofusin 2 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188]

Immunocytochemistry/ Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188]

Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody (S153-5) [NBP2-42188] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.
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Applications for Mitofusin 2 Antibody (S153-5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of Mitofusin 2 Antibody was sufficient for detection of Mitofusin-2 in 20 ug of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Mitofusin 2

MFN2 encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.

Alternate Names

CMT2A, CMT2A2, CPRP1, HSG, MARF, MFN2

Gene Symbol

MFN2

UniProt

Q80U63 (Human)

Additional Mitofusin 2 Products

Product Documents for Mitofusin 2 Antibody (S153-5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mitofusin 2 Antibody (S153-5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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