MKK3/MEK3 Antibody (2E12D11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52508

Novus Biologicals, part of Bio-Techne
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NBP2-52508
NBP2-52508-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2E12D11

Format

BSA Free

Concentration

1 mg/ml

View all MKK3 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human MKK3/MEK3 (AA: 1-138) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKK3/MEK3 Antibody (2E12D11) - BSA Free

Western Blot: MKK3/MEK3 Antibody (2E12D11)BSA Free [NBP2-52508]

Western Blot: MKK3/MEK3 Antibody (2E12D11)BSA Free [NBP2-52508]

Western Blot: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis using MAP2K3 mAb against human MAP2K3 (AA: 1-138) recombinant protein. (Expected MW is 42.1 kDa)
Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Immunohistochemistry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of paraffin-embedded rectum cancer tissues using MAP2K3 mouse mAb with DAB staining.
Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) - BSA Free [NBP2-52508]

Flow Cytometry: MKK3/MEK3 Antibody (2E12D11) [NBP2-52508] - Analysis of Hela cells using MAP2K3 mouse mAb (green) and negative control (red).
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Applications for MKK3/MEK3 Antibody (2E12D11) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry

1:200-1:1000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK3

Mitogen-acitvated protein kinase kinase 3 (MKK3) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK3, closely related to MKK6, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MKK3 is activated by mitogenic and environ-mental stress stimulus as well as by insulin. MKK3 is necessary for the expression of glucose transporter and participates in the MAP kinase- mediated signaling cascade (4).

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

MAP2K3, MEK3, PRKMK3

Gene Symbol

MAP2K3

Additional MKK3 Products

Product Documents for MKK3/MEK3 Antibody (2E12D11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK3/MEK3 Antibody (2E12D11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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