MKK3/MEK3 Antibody (8U2X3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16163

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16163-100ul
NBP3-16163-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8U2X3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MKK3 Antibodies »

Product Summary for MKK3/MEK3 Antibody (8U2X3)

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 150-347 of human MKK3/MEK3 (P46734). FYRKVLDKNMTIPEDILGEIAVSIVRALEHLHSKLSVIHRDVKPSNVLINKEGHVKMCDFGISGYLVDSVAKTMDAGCKPYMAPERINPELNQKGYNVKSDVWSLGITMIEMAILRFPYESWGTPFQQLKQVVEEPSPQLPADRFSPEFVDFTAQCLRKNPAERMSYLELMEHPFFTLHKTKKTDIAAFVKEILGEDS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK3/MEK3 Antibody (8U2X3)

Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Western Blot: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Western blot analysis of extracts of various cell lines, using MKK3/MEK3 Rabbit mAb (NBP3-16163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Immunocytochemistry/Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Immunofluorescence analysis of U-2 OS cells using MKK3/MEK3 Rabbit mAb (NBP3-16163) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Immunocytochemistry/ Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163]

Immunocytochemistry/Immunofluorescence: MKK3/MEK3 Antibody (8U2X3) [NBP3-16163] - Immunofluorescence analysis of C6 cells using MKK3/MEK3 Rabbit mAb (NBP3-16163) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Applications for MKK3/MEK3 Antibody (8U2X3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK3

Mitogen-acitvated protein kinase kinase 3 (MKK3) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). MKK3, closely related to MKK6, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK (3). MKK3 is activated by mitogenic and environ-mental stress stimulus as well as by insulin. MKK3 is necessary for the expression of glucose transporter and participates in the MAP kinase- mediated signaling cascade (4).

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

MAP2K3, MEK3, PRKMK3

Gene Symbol

MAP2K3

Additional MKK3 Products

Product Documents for MKK3/MEK3 Antibody (8U2X3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK3/MEK3 Antibody (8U2X3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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