MKK4/MEK4 Antibody (2 E4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22482

Novus Biologicals, part of Bio-Techne
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NBP2-22482
NBP2-22482-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 2 E4

Concentration

1 mg/ml

View all MKK4 Antibodies »

Product Summary for MKK4/MEK4 Antibody (2 E4)

Immunogen

Protein expressed in 293T cells transfected with human MAP2K4 expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for MKK4/MEK4 Antibody (2 E4)

Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482]

Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482]

Western Blot: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of 50ug whole cell lysate.
Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (2 E4) [NBP2-22482]

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (2 E4) [NBP2-22482]

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (2 E4) [NBP2-22482] - Analysis of MAP2K4 using anti-MAP2K4 monoclonal antibody shows specific expression in HeLa cells (shown in green) but not in negative control NRK cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a mouse monoclonal antibody recognizing MAP2K4, at a dilution of 1:50 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DY-554 phalloidin, nuclei (blue) were stained with Hoechst 33342 dye.

Applications for MKK4/MEK4 Antibody (2 E4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA and 30% glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK4

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Entrez Gene IDs

6416 (Human)

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody (2 E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK4/MEK4 Antibody (2 E4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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