MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72710

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI8A8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MKK4 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Western Blot: MKK4/MEK4 Antibody (OTI8A8)Azide and BSA Free [NBP2-72710]

Western Blot: MKK4/MEK4 Antibody (OTI8A8)Azide and BSA Free [NBP2-72710]

Western Blot: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.
View 6 more images

Applications for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK4

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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