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MKK4/MEK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-99331

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MKK4/MEK4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for MKK4/MEK4 Antibody

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331]

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331]

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody [NBP2-99331] - Immunofluorescence staining of MKK4/MEK4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-HUMAN MKK4/MEK4 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human skeletal muscle with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody [NBP2-99331] - Immunochemical staining of human MKK4/MEK4 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Applications for MKK4/MEK4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunohistochemistry-Paraffin

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK4

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MKK4/MEK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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