MKL2 Antibody (CL1546)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52970

Novus Biologicals, part of Bio-Techne
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NBP2-52970
NBP2-52970-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1546

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MKL2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: QRPGPMELVEKNILPVDSSVKEAIIGVGKEDYPHTQGDFSFDEDSSDALSPDQPASQESQGSAASPSEPKVSESPSPVTTNTPAQFASVSPTVPEFLKTPPTADQPPPRPAAPVLPTNTVSSAKPGPALVKQSHPKNP

Epitope

FASVSPTVPEFLKTP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MKL2 Antibody (CL1546)

Western Blot: MKL2 Antibody (CL1546) [NBP2-52970]

Western Blot: MKL2 Antibody (CL1546) [NBP2-52970]

Western Blot: MKL2 Antibody (CL1546) [NBP2-52970] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970]

Immunocytochemistry/ Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970]

Immunocytochemistry/Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970] - Staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunocytochemistry/ Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970]

Immunocytochemistry/ Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970]

Immunocytochemistry/Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970] - Staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in nuclear speckles and the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Applications for MKL2 Antibody (CL1546)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKL2

MKL2 acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation

Alternate Names

DKFZp686J1745, FLJ31823, FLJ45623, KIAA1243, megakaryoblastic leukemia 2, MKL/myocardin-like 2, MKL/myocardin-like protein 2, MRTFB, MRTF-B, myocardin-related transcription factor B, NPD001

Gene Symbol

MRTFB

Additional MKL2 Products

Product Documents for MKL2 Antibody (CL1546)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKL2 Antibody (CL1546)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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