MKP-1/DUSP1 Antibody (4H7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001843-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4H7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MKP-1 Antibodies »

Product Specifications

Immunogen

DUSP1 (NP_004408, 305 a.a. ~ 367 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LLQFESQVLAPHCSAEAGSPAMAVLDRGTSTTTVFNFPVSIPVHSTNSALSYLQSPITTSPSC

Specificity

DUSP1 - dual specificity phosphatase 1 (4H7)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for MKP-1/DUSP1 Antibody (4H7)

Proximity Ligation Assay: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02]

Proximity Ligation Assay: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02]

Proximity Ligation Assay: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02] - Analysis of protein-protein interactions between MAPK3 and DUSP1. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DUSP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Sandwich ELISA: MKP-1/DUSP1 Antibody (4H7) [H00001843-M02] - Detection limit for recombinant GST tagged DUSP1 is 0.3 ng/ml as a capture antibody.

Applications for MKP-1/DUSP1 Antibody (4H7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity Against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. FACS usage was reported in scientific literature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MKP-1

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq]

Long Name

MAP Kinase Phosphatase 1

Alternate Names

CL100, DUSP1, HVH1, MKP1, PTPN10

Entrez Gene IDs

1843 (Human)

Gene Symbol

DUSP1

UniProt

NP_004408 (Human)

Additional MKP-1 Products

Product Documents for MKP-1/DUSP1 Antibody (4H7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKP-1/DUSP1 Antibody (4H7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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