MLH1 Antibody (MLH1/6284R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13971
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # MLH1/6284R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human MLH1 protein (Uniprot: P40692)
Localization
Nuclear
Specificity
This monoclonal antibody recognizes a protein of 83kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14124)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for MLH1 Antibody (MLH1/6284R)
Western Blot: MLH1 Antibody (MLH1/6284R) [NBP3-13971]
Western Blot: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Western blot analysis of Jurkat cell lysate using MLH1 antibody (MLH1/6284R).Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971]
Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with MLH1 antibody (MLH1/6284R).Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971]
Immunohistochemistry-Paraffin: MLH1 Antibody (MLH1/6284R) [NBP3-13971] - MLH1 antibody (MLH1/6284R). MLH1 Recombinant Rabbit Monoclonal (MLH1/6284R). Inset: PBS instead of primary antibody; secondary only negative control.Applications for MLH1 Antibody (MLH1/6284R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: MLH-1
Additional MLH-1 Products
Product Documents for MLH1 Antibody (MLH1/6284R)
Product Specific Notices for MLH1 Antibody (MLH1/6284R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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