MLX Antibody (PCRP-MLX-1G8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07337

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # PCRP-MLX-1G8

Concentration

0.2 mg/ml

View all MLX Antibodies »

Product Summary for MLX Antibody (PCRP-MLX-1G8)

Immunogen

Recombinant full-length human MLX protein (Uniprot: Q9UH92)

Localization

Nucleus and cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08241)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for MLX Antibody (PCRP-MLX-1G8)

Immunocytochemistry/ Immunofluorescence: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337]

Immunocytochemistry/ Immunofluorescence: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337]

Immunocytochemistry/Immunofluorescence: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337] - Immunofluorescence Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti- Mouse IgG-CF488 (green); counterstain phalloidin (red).
Flow Cytometry: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337]

Flow Cytometry: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337]

Flow Cytometry: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337] - Flow Cytometric Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337]

Protein Array: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337]

Protein Array: MLX Antibody (PCRP-MLX-1G8) [NBP3-07337] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8).

Applications for MLX Antibody (PCRP-MLX-1G8)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: MLX

Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad 1, Mad 3, Mad 4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad 1, Mad 4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

Long Name

MAX-like Protein X

Alternate Names

BigMax alpha, MAD7, MXD7, TCFL4

Gene Symbol

MLX

UniProt

Q9UH92 (Human)

Additional MLX Products

Product Documents for MLX Antibody (PCRP-MLX-1G8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MLX Antibody (PCRP-MLX-1G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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