MLX Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46146

Novus Biologicals, part of Bio-Techne
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NBP1-46146
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all MLX Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QIVKAHQDNPHEGED corresponding to internal region according to NP_937847.1, NP_733752.1.

Epitope

internal region

Specificity

This antibody is expected to recognize all three reported isoforms (NP_937848.1; NP_937847.1; NP_733752.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MLX Antibody

Immunohistochemistry-Paraffin: MLX Antibody [NBP1-46146]

Immunohistochemistry-Paraffin: MLX Antibody [NBP1-46146]

Immunohistochemistry-Paraffin: MLX Antibody [NBP1-46146] - 3.8ug/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for MLX Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 - 3.8 ug/ml

Immunohistochemistry-Paraffin

2.5 - 3.8 ug/ml

Peptide ELISA

Detection limit 1:128000
Application Notes
WB: Preliminary experiments in human, mouse and rat liver lysates gave no specific signal but low background. IHC-P: Human placenta shows nuclear stsaining of trophoblasts.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MLX

Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad 1, Mad 3, Mad 4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad 1, Mad 4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

Long Name

MAX-like Protein X

Alternate Names

BigMax alpha, MAD7, MXD7, TCFL4

Entrez Gene IDs

6945 (Human); 21428 (Mouse); 360631 (Rat)

Gene Symbol

MLX

UniProt

NP_937848.1 (Human)

Additional MLX Products

Product Documents for MLX Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MLX Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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