MMAB Antibody (OTI2G5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72730

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MMAB Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MMAB (NP_443077) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMAB Antibody (OTI2G5) - Azide and BSA Free

Western Blot: MMAB Antibody (OTI2G5)Azide and BSA Free [NBP2-72730]

Western Blot: MMAB Antibody (OTI2G5)Azide and BSA Free [NBP2-72730]

Western Blot: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB.
Immunohistochemistry: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730]

Immunohistochemistry: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730]

Immunohistochemistry: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730] - Staining of paraffin-embedded Human liver tissue using anti-MMAB mouse monoclonal antibody.
Flow Cytometry: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730]

Flow Cytometry: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730]

Flow Cytometry: MMAB Antibody (OTI2G5) - Azide and BSA Free [NBP2-72730] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MMAB antibody, and then analyzed by flow cytometry.
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Applications for MMAB Antibody (OTI2G5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMAB

MMAB encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. [provided by RefSeq]

Alternate Names

aquocob(I)alamin vitamin B12s adenosyltransferase, ATP:cob(I)alamin adenosyltransferase, ATP:corrinoid adenosyltransferase, ATR, cblB, c-diamide adenosyltransferase, mitochondrial, cob, Cob(I)alamin adenosyltransferase, cob(I)yrinic acid a, EC 2.5.1.17, methylmalonic aciduria (cobalamin deficiency) cblB type, methylmalonic aciduria (cobalamin deficiency) type B, Methylmalonic aciduria type B protein, MGC20496

Gene Symbol

MMAB

Additional MMAB Products

Product Documents for MMAB Antibody (OTI2G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMAB Antibody (OTI2G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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