MMAB Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86602

Novus Biologicals, part of Bio-Techne
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NBP1-86602
NBP1-86602-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MMAB Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AFILPSGGKISSALHFCRAVCRRAERRVVPLVQMGETDANVAKFLNRLSDYLFTLARYAAMKEGNQEKIYMKNDPSAESEGL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (84%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMAB Antibody

Western Blot: MMAB Antibody [NBP1-86602]

Western Blot: MMAB Antibody [NBP1-86602]

Western Blot: MMAB Antibody [NBP1-86602] - Analysis in human cell line MCF-7 and human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: MMAB Antibody [NBP1-86602]

Immunocytochemistry/ Immunofluorescence: MMAB Antibody [NBP1-86602]

Immunocytochemistry/Immunofluorescence: MMAB Antibody [NBP1-86602] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: MMAB Antibody [NBP1-86602]

Immunohistochemistry-Paraffin: MMAB Antibody [NBP1-86602]

Immunohistochemistry-Paraffin: MMAB Antibody [NBP1-86602] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
View 3 more images

Applications for MMAB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMAB

MMAB encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. [provided by RefSeq]

Alternate Names

aquocob(I)alamin vitamin B12s adenosyltransferase, ATP:cob(I)alamin adenosyltransferase, ATP:corrinoid adenosyltransferase, ATR, cblB, c-diamide adenosyltransferase, mitochondrial, cob, Cob(I)alamin adenosyltransferase, cob(I)yrinic acid a, EC 2.5.1.17, methylmalonic aciduria (cobalamin deficiency) cblB type, methylmalonic aciduria (cobalamin deficiency) type B, Methylmalonic aciduria type B protein, MGC20496

Entrez Gene IDs

326625 (Human)

Gene Symbol

MMAB

UniProt

Q96EY8 (Human)

Additional MMAB Products

Product Documents for MMAB Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MMAB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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