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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 39-296 of human MMADHC (NP_056517) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMADHC Antibody (OTI1G4) - Azide and BSA Free

Western Blot: MMADHC Antibody (OTI1G4)Azide and BSA Free [NBP2-72739]

Western Blot: MMADHC Antibody (OTI1G4)Azide and BSA Free [NBP2-72739]

Western Blot: MMADHC Antibody (OTI1G4) - Azide and BSA Free [NBP2-72739] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMADHC.
Western Blot: MMADHC Antibody (OTI1G4)Azide and BSA Free [NBP2-72739]

Western Blot: MMADHC Antibody (OTI1G4)Azide and BSA Free [NBP2-72739]

Western Blot: MMADHC Antibody (OTI1G4) - Azide and BSA Free [NBP2-72739] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for MMADHC Antibody (OTI1G4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMADHC

MMADHC encodes a mitochondrial protein that is involved in an early step of vitamin B12 metabolism. Vitamin B12 (cobalamin) is essential for normal development and survival in humans. Mutations in this gene cause methylmalonic aciduria and homocystinuria type cblD (MMADHC), a disorder of cobalamin metabolism that is characterized by decreased levels of the coenzymes adenosylcobalamin and methylcobalamin. Pseudogenes have been identified on chromosomes 11 and X.

Alternate Names

cblD, chromosome 2 open reading frame 25, methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria, mitochondrial, protein C2orf25, mitochondrial

Gene Symbol

MMADHC

Additional MMADHC Products

Product Documents for MMADHC Antibody (OTI1G4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MMADHC Antibody (OTI1G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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