MMP-12 Antibody (2G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26138

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2G3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-12 Antibodies »

Product Summary for MMP-12 Antibody (2G3)

Immunogen

A synthesized peptide derived from Human MMP-12 [UniProt P39900]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-12 Antibody (2G3)

MMP-12 Antibody (2G3)

Immunohistochemistry: MMP-12 Antibody (2G3) [NBP3-26138] -

Immunohistochemistry: MMP-12 Antibody (2G3) [NBP3-26138] - Image of MMP-12 Antibody (2G3) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
MMP-12 Antibody (2G3)

Western Blot: MMP-12 Antibody (2G3) [NBP3-26138] -

Western Blot: MMP-12 Antibody (2G3) [NBP3-26138] - Positive Western Blot detected in: 293T whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate.
All lanes: MMP-12 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 55 kDa
Observed band size: 54, 45 kDa

Applications for MMP-12 Antibody (2G3)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: MMP-12

Human macrophage elastase (MMP-12) is a member of the family of matrix metalloproteinases (MMPs) that plays, like other members of the family, an important role in inflammatory processes contributing to tissue remodeling and destruction. In particular, a prominent role of MMP-12 in the destruction of elastin in the lung alveolar wall and the pathogenesis of emphysema has been suggested. It is therefore an attractive therapeutic target (1). MMP-12) expressed mainly in alveolar macrophages has been identified in the mouse lung as the main destructive agent associated with cigarette smoking, which gives rise to emphysema, both directly via elastin degradation and indirectly by disturbing the proteinase/antiproteinase balance via inactivation of the alpha1-proteinase inhibitor (alpha1-PI), the antagonist of the leukocyte elastase (2). Because elastin represents a critical component of aortic wall structure and a matrix substrate for metalloelastases, MMP-12 may have a direct and singular role in the pathogenesis of aortic aneurysms (3).

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Gene Symbol

MMP12

Additional MMP-12 Products

Product Documents for MMP-12 Antibody (2G3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-12 Antibody (2G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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