MMP-13 Antibody (JF0893)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66954

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-66954
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF0893

Concentration

1 mg/ml

View all MMP-13 Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human MMP-13 aa 390-430. (SwissProt: P45452 Human)

Localization

Extracellular matrix, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-13 Antibody (JF0893)

Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954]

Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954]

Western Blot: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of MMP13 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SW480 cell lysate Lane 2: MCF-7 cell lysate
Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954]

Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954]

Immunocytochemistry/Immunofluorescence: MMP-13 Antibody (JF0893) [NBP2-66954] - Staining MMP13 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954]

Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954]

Immunohistochemistry-Paraffin: MMP-13 Antibody (JF0893) [NBP2-66954] - Analysis of paraffin-embedded human lung cancer tissue using anti-MMP13 antibody. Counter stained with hematoxylin.
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Applications for MMP-13 Antibody (JF0893)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MMP-13

The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders.

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Gene Symbol

MMP13

Additional MMP-13 Products

Product Documents for MMP-13 Antibody (JF0893)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-13 Antibody (JF0893)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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