MMP-13 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-45723

Novus Biologicals, part of Bio-Techne
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NBP1-45723SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Equine, Rabbit

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all MMP-13 Antibodies »

Product Specifications

Immunogen

Synthetic peptide surrounding amino acid 454 of rat MMP-13.

Specificity

The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-13 Antibody

Western Blot: MMP-13 Antibody [NBP1-45723]

Western Blot: MMP-13 Antibody [NBP1-45723]

Western Blot: MMP-13 Antibody [NBP1-45723] - Analysis of MMP-13 expression with Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody [NBP1-45723]

Immunocytochemistry/ Immunofluorescence: MMP-13 Antibody [NBP1-45723]

Immunocytochemistry/Immunofluorescence: MMP-13 Antibody [NBP1-45723] - MMP-13 antibody was tested at 1:50 in MDA-MB-231 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40X.
Western Blot: MMP-13 Antibody [NBP1-45723]

Western Blot: MMP-13 Antibody [NBP1-45723]

Western Blot: MMP-13 Antibody [NBP1-45723] - Analysis of MMP-13 in human full-length recombinant protein using anti-MMP-13 antibody. Image from verified customer review.
View 1 more images

Applications for MMP-13 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 25703139).

Reviewed Applications

Read 1 review rated 3 using NBP1-45723 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2), 5 mM EDTA, 0.5% BSA, 30% Glycerol

Preservative

0.03% Proclin 300

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MMP-13

Collagenase-3 (MMP13), a member of the matrix metalloproteinase (MMP) family of neutral endopeptidases, is expressed in the skeleton during embryonic development and is highly overexpressed in human carcinomas and in chondrocytes and synovial cells in rheumatoid arthritis and osteoarthritis. Findings demonstrate a unique role of Mmp13 in skeletal development (1). In arthritis, the destruction of fibrillar (type II) collagen is one of the hallmarks, with MMP-1 and MMP-13 being identified as key players in arthritic cartilage. MMP-13, furthermore, has been found in highly metastatic tumors. In contrast to many human MMPs, the MMP-13 distribution pattern is restrictive in normal tissues and selective in pathological conditions. MMP-13 demonstrates versatility in its substrate utilization. In addition to being highly active on type II collagen, MMP-13 cleaves other substrates, mostly macromolecules of the extracellular matrix, but also molecules such as connective tissue (CTGF) and fibrinogen. MMP-13 is controlled at multiple levels: i.e., the expression/synthesis, activation, and inhibition of the active enzyme (3).

Long Name

Matrix Metalloproteinase 13

Alternate Names

MMP13

Entrez Gene IDs

4322 (Human); 17386 (Mouse); 171052 (Rat); 281914 (Bovine)

Gene Symbol

MMP13

UniProt

P23097 (Rat)

Additional MMP-13 Products

Product Documents for MMP-13 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MMP-13 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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