Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3G4.2

Format

BSA Free

Concentration

1 mg/ml

View all MMP-14/MT1-MMP Antibodies »

Product Specifications

Immunogen

Recombinant protein containing the catalytic domain of human MT1-MMP.

Reactivity Notes

Human

Specificity

Clone 3G4.2 recognizes the catalytic domain of MT1-MMP, identifying the active form of the protein. MW is approximately 60 kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_004995.2

Scientific Data Images for MMP-14/MT1-MMP Antibody (3G4.2) - BSA Free

Western Blot: MMP-14/MT1-MMP Antibody (3G4.2)BSA Free [NBP2-29735]

Western Blot: MMP-14/MT1-MMP Antibody (3G4.2)BSA Free [NBP2-29735]

Western Blot: MMP-14/MT1-MMP Antibody (3G4.2) - BSA Free [NBP2-29735] - U251 cell lysate was transfected with MT1-MMP and separated on 10% gel(40 ug total protein/lane). A representative lot of Anti-MT1-MMP, clone 3G4.2, at 1:200 recognizes a 60 kDa band, the active form of MT1-MMP.

Applications for MMP-14/MT1-MMP Antibody (3G4.2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MMP-14/MT1-MMP

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP-14 is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. MT1-MMP is capable of mediating pericellular proteolysis of extracellular matrix components and is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix. This protein also activates MMP2 protein, and this activity may be involved in tumor invasion.

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

UniProt

P50281

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (3G4.2) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MMP-14/MT1-MMP Antibody (3G4.2) - BSA Free

Relevant Entrez Gene Numbers: NM_004995.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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