MMP-14/MT1-MMP Antibody (6T7L3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15247

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15247-100ul
NBP3-15247-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6T7L3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-14/MT1-MMP Antibodies »

Product Summary for MMP-14/MT1-MMP Antibody (6T7L3)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MMP-14/MT1-MMP (P50281). GAEIKANVRRKRYAIQGLKWQHNEITFCIQNYTPKVGEYATYEAIRKAFRVWESATPLRFREVPYAYIREGHEKQADIMIFFAEGFHGDSTPFDGEGGFLA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-14/MT1-MMP Antibody (6T7L3)

Western Blot: MMP-14/MT1-MMP Antibody (6T7L3) [NBP3-15247]

Western Blot: MMP-14/MT1-MMP Antibody (6T7L3) [NBP3-15247]

Western Blot: MMP-14/MT1-MMP Antibody (6T7L3) [NBP3-15247] - Western blot analysis of extracts of various cell lines, using MMP14/MMP-14/MT1-MMP Rabbit mAb (NBP3-15247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western Blot: MMP-14/MT1-MMP Antibody (6T7L3) [NBP3-15247]

Western Blot: MMP-14/MT1-MMP Antibody (6T7L3) [NBP3-15247]

Western Blot: MMP-14/MT1-MMP Antibody (6T7L3) [NBP3-15247] - Western blot analysis of extracts of various cell lines, using MMP14/MMP-14/MT1-MMP Rabbit mAb (NBP3-15247) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for MMP-14/MT1-MMP Antibody (6T7L3)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (6T7L3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-14/MT1-MMP Antibody (6T7L3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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