MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-60993

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MM0027-9E10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MMP-14/MT1-MMP Antibodies »

Product Specifications

Immunogen

Human recombinant MMP-14/MT1-MMP

Localization

Membrane; Single-pass type I membrane protein. Melanosome.

Specificity

This antibody recognizes human MMP-14 in western blot without cross reactivity with human MMP-1-13, and MMP14-15.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free [NB110-60993]

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free [NB110-60993]

Immunohistochemistry-Paraffin: MMP14 Antibody (MM0027-9E10) [NB110-60993] - Human placenta (formalin fixed and paraffin embedded) section is subjected to IHC.

Applications for MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:400-1:800

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. MMP14 may be an activator of pro gelatinase A and is expressed in fibroblast cells during both wound healing and human cancer progression. MMP14 is expressed in very low levels and may require stimulation with concanavolin A or the phorbol ester TPA to stimulate production of MMP14.

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Entrez Gene IDs

4323 (Human)

Gene Symbol

MMP14

UniProt

P50281

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMP-14/MT1-MMP Antibody (MM0027-9E10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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