Key Product Details

Species Reactivity

Human, Mouse, Rat, Guinea Pig

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-2 Antibodies »

Product Specifications

Immunogen

Activated recombinant human MMP2. [UniProt# P08253]

Specificity

This is specific for pro and active MMP-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for MMP-2 Antibody (2C1) [DyLight 405]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: MMP-2

MMP2 (matrix metalloproteinase-2; CLG4A) is a ubiquitinous metalloproteinase enzyme belonging to peptidase M10A family which is implicated in versatile activities including vasculature remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. Besides degrading proteins in the extracellular matrix, MMP2 also acts on various nonmatrix proteins such as big endothelial 1, beta-type CGRP promoting vasoconstriction, KISS etc. MMP2 plays a key role in myocardial cell death pathways and also contributes to myocardial oxidative stress through regulation of GSK3-beta activity. PKC facilitates MDM2 phosphorylation which is crucial to its enzymatic activity. MMP2 propeptide is processed by MMP14/MT-MMP1 and MMP16/MT-MMP3, and a C-terminal autocatalytic cleavage generates PEX chain which exerts anti-angiogenic as well as anti-cancer properties alongwith inhibiting cell migration or cell adhesion to FGF2/vitronectin. MMP2's isoform 2 facilitates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of pro-inflammatory NFkB, NFAT and IRF transcriptional pathways. MMP2 is implicated in several pathologies including metastasis of cancer and defects in MMP2 have been linked to Torg-Winchester syndrome (TWS) which is also called as multicentric osteolysis nodulosis and arthropathy (MONA).

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for MMP-2 Antibody (2C1) [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MMP-2 Antibody (2C1) [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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