MMP-2 Antibody (3Q7R10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15653

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15653-100ul
NBP3-15653-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3Q7R10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MMP-2 Antibodies »

Product Summary for MMP-2 Antibody (3Q7R10)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human MMP-2 (P08253). RDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MMP-2 Antibody (3Q7R10)

Western Blot: MMP-2 Antibody (3Q7R10) [NBP3-15653]

Western Blot: MMP-2 Antibody (3Q7R10) [NBP3-15653]

Western Blot: MMP-2 Antibody (3Q7R10) [NBP3-15653] - Western blot analysis of extracts of various cell lines, using MMP-2 antibody (NBP3-15653) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for MMP-2 Antibody (3Q7R10)

Application
Recommended Usage

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMP-2

MMP2 is a member of the Matrix Metalloproteinase (MMP) family, which are involved in extracellular matrix degradation under both normal physiological and disease processes. Specifically, MMP-2 is responsible for degrading collagens type IV, V, VII, and X and gelatin type I. Other reported functions of MMP 2 include angiogenesis, tumor invasion, tissue repair, remodeling of the vasculature, inflammation, and atherosclerotic plaque rupture. MMP-2 secretion is activated by the Ras signaling pathway. MMP2 triggers cells to begin migration by cleaving laminin-5 and exposing an integrin-binding site on the epithelial basement membrane. The cleaved of laminin has been detected in tumors and in tissues being altered, howver the activated form of MMP2 is not found in benign tumors. Thus, detection of MMP2 is a possible early indicator of tumor activity. MMP2 is most strongly expressed in normal skin fibroblasts, but may also be found in gliomas, breast and prostate tumors. Mutations in the MMP-2 gene have been linked to Torg-Winchester syndrome, Nodulosis-Arthropathy-Osteolysis (NAO) syndrome, and arthritis. Additionally, abnormal expression of matrix matalloproteinases have been associated with neoplastic traits such as loss of negative growth regulation and high invasive potential.

Long Name

Matrix Metalloproteinase 2

Alternate Names

Gelatinase A, MMP2

Gene Symbol

MMP2

Additional MMP-2 Products

Product Documents for MMP-2 Antibody (3Q7R10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MMP-2 Antibody (3Q7R10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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